Environmental bio-monitoring with high-throughput sequencing

Environmental bio-monitoring with high-throughput sequencing

There is much interest in using high-throughput DNA sequencing methodology to monitor microorganisms, complex plant and animal communities. However, there are experimental and analytical issues to consider before applying a sequencing technology, which was originally developed for genome projects, to ecological projects. Many of these issues have been highlighted by recent microbial studies. Un...

Detecting and monitoring lymphoma with high-throughput sequencing

(2011). IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients. Oncotarget, 2(3), 178-185. Each mature T cell or B cell has an allele with a productively rearranged T cell receptor (TCR) or B cell receptor (BCR) gene, respectively. The repertoire of TCRs and BCRs in the blood of a healthy person is diverse, with no single specific rearrangement found in more than one pe...

High-throughput sequencing technologies.

The human genome sequence has profoundly altered our understanding of biology, human diversity, and disease. The path from the first draft sequence to our nascent era of personal genomes and genomic medicine has been made possible only because of the extraordinary advancements in DNA sequencing technologies over the past 10 years. Here, we discuss commonly used high-throughput sequencing platfo...

SnapShot: High-Throughput Sequencing Applications

High-throughput, high-fidelity HLA genotyping with deep sequencing.

Human leukocyte antigen (HLA) genes are the most polymorphic in the human genome. They play a pivotal role in the immune response and have been implicated in numerous human pathologies, especially autoimmunity and infectious diseases. Despite their importance, however, they are rarely characterized comprehensively because of the prohibitive cost of standard technologies and the technical challe...